Part:BBa_K1679037:Design
VMA Dead, an inactivated intein
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
VMA Dead is a inactivated mutation of VMA, we constructed it by replace Asn-464 with Gln. We design this part as a control group of VMA. We constructed this part from VMA Dead and its exteins in original host. This VMA Dead part is an inactivated intein with 10 residues of native N-exteins and C-exteins of VMA at the junction. While fusing intein to protein, users can fuse the intein with it's flanking residues (we recommand 2~3 residues).
Source
We mutated a amino acid of VMA by PCR to get this part.
References
Liang R, Liu X, Liu J, et al. A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli[J]. Journal of microbiological methods, 2007, 68(3): 497-506.
Ellilä S, Jurvansuu J M, Iwaï H. Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli.[J]. Febs Letters, 2011, 585(21):3471-3477.